Vol. 3 No. 1 (2024): The QUEST: Journal of Multidisciplinary Research and Development
Articles

Molecular Detection of Extended Spectrum β-lactamase (ESBL) producing Escherichia coli from Chicken (Gallus gallus domesticus) meat isolated from Public Markets in Cabanatuan City, Nueva Ecija

  • Paul Joshua Balosbalos,
  • Rachelle Conmigo,
  • Lois Pamella Tulio,
  • Angelika Mendoza,
  • Diana Rose Mahilaga,
  • Diana Elizabeth Cortez,
  • Danica Dimacale
Paul Joshua Balosbalos
Nueva Ecija University of Science and Technology
Rachelle Conmigo
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
Lois Pamella Tulio
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
Angelika Mendoza
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
Diana Rose Mahilaga
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
Diana Elizabeth Cortez
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
Danica Dimacale
Biology Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology
DOI: https://doi.org/10.60008/thequest.v3i1.123

Published 06/30/2024

Keywords

  • Antibiotic Resistance,
  • Chicken meat,
  • E. coli,
  • Extended-spectrum β-lactamase,
  • blaCTX-M

How to Cite

Balosbalos, P. J., Conmigo, R., Tulio, L. P., Mendoza, A., Mahilaga, D. R., Cortez, D. E., & Dimacale, D. (2024). Molecular Detection of Extended Spectrum β-lactamase (ESBL) producing Escherichia coli from Chicken (Gallus gallus domesticus) meat isolated from Public Markets in Cabanatuan City, Nueva Ecija. The QUEST: Journal of Multidisciplinary Research and Development, 3(1). https://doi.org/10.60008/thequest.v3i1.123

Copyright (c) 2024 The QUEST: Journal of Multidisciplinary Research and Development

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Abstract

Antibiotic resistance poses a considerable threat to human populations, especially those belonging to developing countries, like the Philippines. The high prevalence of improper antimicrobial usage has led to the rise of multi-drug resistant microorganisms, harboring genes, such as blaCTX-M, responsible for the proliferation of microorganisms with extrinsic resistance to β-lactam antibiotics. This study investigated the presence of ESBL-producing Escherichia coli isolated from commercially available chicken meat sold in Public Markets from Cabanatuan City. Phenotypic confirmation of the species was done via macroscopic methods (culture) using selective and differential media supplemented with 1mg/L cefotaxime. Bacterial genomic DNA extraction using boil lysis method was done prior to PCR amplification using a primer for blaCTX-M to confirm resistance attributed to β-lactamase production. Nine (9) isolates were characterized, depicting dark colonies with green-metallic sheen in Eosin-Methylene Blue agar as E. coli. The presence of blaCTX-M gene for ESBL-producing E. coli was detected across all isolates based on the ~592bp bands from the PCR amplification. The results of this study encompasses the need for surveillance of AMR, highlighting the significance of the risk posed by these resistant species present in food products. Further testing is recommended to fully characterize the isolates’ resistance profiles and genotypes to further assess their geographical distribution.

Full Paper

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